Weeeeeeeeeell do you think of yourself as female?
Aaaaaaaand are they aware of it?
I've had a couple of friends that just weren't connecting the damned dots. "give me your man card" in reply to something I had said, and me replying "I don't have a man card you idiot".
Part of the process of my realization was coming to look back with hindsight at myself and finally understand so much of who I was, that all along I had never really understood.
I am not even capable of being offended when mistaken as female. Duh

The other day while walking home from my visit, this guy walks past real close from exiting the town hall, "Good day sir" rather friendly like, "how are you today sir?". So I greeted him back, but part of me wanted to say, 'you know we have some great optometrists in town, you should visit one'. I mean what part of my purse, carried like a purse, my jewellery and just abut everything about my behaviour was invisible?
He was just being friendly, but, it still irked me.
Doofus, are you blind?