Hey, Kate;
I really like your recent post on identity/physical/legal. When I first accepted my transsexuality and with that, realized I need to transition, I too searched for the universal things that made one female or feminine. I tried to learn 'the walk', tried to do the knee-over-knee crossing the legs (rather than the male figure-four,) etc.
Then I realized a lot of Natal females didn't walk in any particular manner and, when not wearing a skirt (which is actually kind of rare these days,) would cross their legs figure-four style.
Yet, there's still some 'essence' of femaleness that comes through.
A few months ago I bought the Denae Doyle videos. I found they were only narrowly useful -- say, if I was going to attend a formal ball or other event where I had to behave in a highly stylized female manner, but kinda' useless for everyday mannerisms.
I think the biggest difference that happened was upon truly accepting myself I stopped monitoring my mannerisms and censoring anything that wasn't 'masculine'. Now I stand/walk/sit the way I feel like. The old me still sometimes wells up and tries to say "Stop That!", but I just smile to myself and let it happen.
Somewhere else was a topic on what to wear to a support group meeting. My answer to me is, 'Wear what you feel comfortable with.' With my therapists' concurrence, I'm actually playing a mental game with myself wherein I'm a female who is -- "deep stealth" style -- presenting as a guy (or trying to

) As my hair grows out longer, as electrolysis steadily erodes my facial hair, as HRT causes my breasts to develop further, redistributes body fat, and softens body hair and skin, and more importantly, the mental attitues & such become more profoundly changed, it'll be harder and harder to present as a guy. When strangers begin to refer to me as female, I'll know that Karen's undercover assignment to spy on the other team is coming to an end.
Now all the above really does for me is take the pressure off to 'behave' in a 'feminine' manner, and just behave as me. The female-ness will still shine through and, over time, will only become stronger.
Still, that doesn't answer the topic question. What makes 'Female'? An appreciation for the quality of 'cute'? An empathetic sense of what others might be feeling? I find there's a feeling that's hard to put into words -- I can only describe facets of it. I can tap into this feeling and use it when making videos to fine-tune the emotional undercurrent, enhancing the videos' effectiveness. I can't explain why I did it that way, but somehow I just -- know...
Sorry for the vague and amorphous post. I hope it somehow adds to this discussion.
Karen