Occasionally when I was younger pepole would accuse me of begin a girl mannerisms and androgyny and what not sadly puberty put a stop to most of that.
I went to a friends house at one point an my friends mother flatly refused to believe I was a boy. She described me perfectly but was insistent even to my friend that I was a girl. I was quietly flattered. And because I'm bashful when complimented anyway, my response was construed as embarrassment.
Other cases have resulted in more direct accusations about whether I have gender issues.
Once was from an ex-girlfriend I denied it at the time and lied thus, at the time the relationship was fine and I didn't want to potentially endanger it by worrying her with this fact. the body dysphora did infact get in the way and contributed to ending the relationship.
Another time was from my best friend who I've known since childhood, he's not really very empathic so I'm not that surprised it took him like 10 years to notice, I did admit to him partly because there is the security there that if he acts like an ass with it I can always wreck his life with equally devastating confided information.
He still responds very inappropriately and gets the wrong idea alot of the time with said knowledge, But that's a different story.
I also got a similar question from my sister... To which I responded ambiguously but was ultimately a "yes"
Her reactions to me have changed alittle she is sympathetic, but things are always kinda strange with her because despite having near identical life experiences our personalities and behaviours are near polar opposites. So I think it confuses her how she should react to such knowledge.
Never had any such insinuation from my parents however. I rarely see my dad but year my mum certainly expects me to be a "man" or whatever that's supposed to be and I find myself compelled to act to her expectation. I general I just don't want to let pepole down and that's what's preventing me moving forward.