The difference between typical male and female inflection has been touched on already in this thread... but there's one other difference in speech patterns that ought to be mentioned: men give orders; women try to get other people to like them.
If you listen to typical male speech patterns, they're very direct, to-the-point and decisive. He's not trying to get you to like him or (necessarily) agree with him; he's simply asserting himself and telling you how it is. If necessary, he'll use humour to deflect any apparent offence.
Typical female speech patterns use a choice of words, tone of voice, and questioning intonation to make them sound a little unsure of what they're saying because they don't want to hurt your feelings; their main aim is to get you to like them so you'll agree with them. If they try to use humour, it often falls flat.
So the typical male speech pattern is very matter-of-fact and the female speech pattern is rather more emotional.
This has always been a problem for me because I've always had a very typical male speech pattern (due to my Asperger's) which meant that people who saw me as female would find me quite rude. But in actual fact I was much more polite than my brother, who wasn't seen as rude.
When it comes to mannerisms: when women walk they lead from the chest; when men walk they lead from the hips and they assume more space for themselves than women do. You'll know you're getting it right when you notice that women practically walk into you - it'll mean they're presuming you'll get out of their way like most guys do.