Yes and no, which might not have seemed possible as an answer.
I pretty quickly lost the reflex to protect my nonexistent testicles... but I equally quickly developed the urge to shield a slightly different area, helped along by what I have affectionately nicknamed the "clitoris-stomping cat." It's basically impossible to get hit in the vagina and of course the area where testicles were is empty now, but that doesn't mean there aren't still some tender bits (a solid hit to the pubic bone also hurts like heck).
Of course, now it's hard to shield the nipples at the same time!
(I suppose the cat is getting her revenge - she used to like to use those bits as a shelf to stand on, and now she keeps trying to and falling through into my lap instead.)