It depends what you want. I was an RA supervisor in college and grad school, and saw bunches of people opt for single rooms for various reasons. I also saw other people under the same conditions choose to live with roommates. What kind of college experience are you looking for? That's what it really boils down to.
Personally, I would opt for a single room however I could get one. But that's because I like to have privacy and my own space. I would also have no problem sharing with other guys if that was the only option available. But if just the thought makes you uncomfortable, I'm not sure I can encourage you to go that route.
I will say from professional experience that most roommate issues are behavior related and not personal. If they do not want to live with a transguy/a gay guy/a black guy/etc. it is something that will immediately come up when you move in.
Something you could check on is if they have any specific "communities" available. It's becoming popular at a lot of schools to designate floors/buildings to students of a certain major, lifestyle, social interest, etc. My freshman year I lived on a floor dedicated to sustainability. As an RA, I ran 2 floors related to my major and another for LGBTQA students. If they have something like that available, see if you can get in there. It's obviously not a guarantee that things would go well, but it would probably feel more comfortable than going in blind.