Mine has become somewhat (probably more than somewhat) prettier, a bit more rounded and so on. That said, my original handwriting was a complete mess, so it's still not what I would call pretty. I also at times have hand tremor because of medicines, which obviously affects handwriting. But all in all, yes, it has changed. I have no idea how people would "gender" my handwriting (which is pure and utter nonsense anyways, you can't determine gender from handwriting), I guess it would depend on the person seeing it and also on my mood, degree of hand tremor and whether or not I was in a hurry at the time of writing.
Also, in general, I rarely write by hand at all except when signing stuff or in rare cases filling in forms which can't be filled in electronically. Oh, and finals at university, but even then I use a computer for everything except math, electronics (drawing circuits in MS Word is kind of a hassle) and other courses where the test isn't text-centered. I can go weeks without touching a pen in extreme cases. I suspect frequency of handwriting also plays a huge part - if I wrote more stuff by hand, I'd probably also get better at it.