I don't know how much of the changes in the way people treat me in public has changed because of the area I now live in, or because people are reading me as male. Although I've always presented the way I do and I've almost always been read as male, I used to live in a small village in the countryside, so everyone knew I was "supposed" to be female. Now I'm living in town that's not particularly friendly (in the first month I lived here a pensioner was stabbed 47 times about 100 yards from my work), and I'm always read correctly as male because they don't know I've ever been thought of as anything else. I don't know whether these changes are because they think I'm male, or because it's an entirely different culture.
As I was moving into my flat there was a woman smoking outside the entrance to the building. My mother was there and she told me I had to say hello to her as she was presumably a neighbour, so I tried saying "Good evening" to her, and she ended up walking off and hiding behind the garages.
When I'm cycling down one of the paths I often pass people, and always thank them for stepping out of the way (I'm not just ignorant, it's an actual cycle path), but I just get glares at most in response.
People don't tend to sit next to me on public transport, but that suits me perfectly, if it's full-ish when I get on I choose to stand rather than sit next to someone.
I've been asked for a cigarette once on my way to work, which I thought was odd as most people read me as about 14, but then again it could be the area. I wasn't even smoking at the time, and don't think I look like a stereotypical smoker. I was never asked when people thought I was female.