Kiera Knightly looks almost skeletal in a lot of movies. I think the undead are non-binary.

I think Lestat and Lui in an interview with a vampire where particulalry non-binary.
Oh I have a good one - the angels in Dogma i.e. Matt Daimon etc.. They did not seem particulalry non-binary but they had no private parts - literarly not male or female if genetalia is the yard stick.
In Deepspace Nine they have that weird morphing security officer - Otto?. I'm pretty sure they are a non-binary character as they could conceivably be anything.
I could go on a bit of a limb here - but I always saw Jesus in movies as a character that transended mere male or female. I know in christianity he was specifically male, but he seemed much more than just man (yet at the same time he was particulalry human in nature).