I was at the NYC public library last weekend visiting a gg friend and as we were leaving the woman working security called us ladies and asked to check our bags on the way out. This has happened several times before but usually there is some back pedaling when they take a closer look, but not this time.
I did spend the next day presenting female and we got called "ladies" several times. I find that it is way more common to use gender specific articles like ma'am in the US (I got ma'amed 4 times when I went to Florida last Christmas when presenting male) than here in Canada, at least when speaking English, French is a different story.
Jenna!