I'm a bit confused by this topic since it's in the AG section. I guess I wonder what you presented as during that situation; gender neutral, gender queer, male, female?... Because if it was either of the first two then I'm not sure why you'd become angry about those comments - I mean, obviously you achieved your goal; they couldn't figure you out. Sure, 'it' can be quite a boo-hoo word, but it's not like you can really expect folk to suddenly know different terms besides 'male' and 'female' or have a booklet on neutral pronouns. If you look at it that way, 'it' probably wasn't even the most 'negative' choice.
For a bit of personal input: I present myself in a gender-neutral fashion and people often have trouble determining my biological sex (from a distance they often figure me male and then get confused when I come closer). Every now and then they whisper, sometimes I get a 'Sorry, but I really can't see whether you're trying to be Rose or Roy' comment - mostly people tend to be intrigued and I get hit on by high school girls. It doesn't make me angry, it makes me want to do a little dance and pull off a cocky little smirk because it means I am read the way I see myself on the inside (or better put: lacked to read). The only comment that annoys me is '(bull)dyke' and I admit the highschool girls make me somewhat uncomfortable in a 'I'm really too old for you' kind of way.