The other side of the coin from the favorite things thread.
I'll start...

1. Getting up 1 hour earlier to do my make-up, hair, clothes before work every day.
2. Attention from guys. It's cool and affirming at first, but gets old fast. If another dude yells some cheesy pickup line at me from the window of their car while I'm out running or hits on me in the grocery store by pretending to need help finding stuff... I'll scream.
3. Getting treated like a 5 year old child... by mechanics, repair guys, or even some guys on dates that go: "Do you know how the game of football works? Do you even know how to keep score?" Then proceed to start explaining it to me like I'm their 5 year old child... hell I played the game for 6 years... I have an idea. Girls don't ever follow sports?
4. Women's Restrooms. They are dirtier and more crowded overall. The stalls are usually all occupied, seats wet/dirty and you find the strangest things lying around on the floor. Also it's like a club where women like to hang-out and talk at the sink/mirror. No more going in, doing your deed, and leaving. It's now a social event.
5. Money. It costs a whole lot more to be a girl. Clothes, make-up, hair stuff, underwear, et al are all stuff you have to spend a lot more money on.
6. Maintenance. It takes a whole lot more work to look "presentable" as a female. No more rolling out of bed, not fixing/combing my hair, throwing on any old t-shirt and shorts and walking out the door.
7. Becoming a member of the secret "women's club" where if you are alone in a room with another woman and 5 guys... the one woman will always want to start a conversation with me and suddenly want to be my best friend. Hair, clothes, make-up, whatever... sometimes people will find the most common and innocuous stuff to start a long boring conversation about.
8. Perception and stereotypes. Just because you may look like the average 20s-something chick doesn't mean I automatically like all the same things every other 20s-something chick does... like music, fun stuff to do, clubs and expectations. Everyone is different, and a book doesn't always match it's cover. Every person is different.