Yep. My wife and I had been together for well over a decade when I transitioned, so I *know* nothing changed about how we treat each other in public (and we're not into PDA, so I'm talking leaning on each other, hand-holding, the occasional quick peck on the lips), and yet now we get a lot more second looks than we did before. And it seems like the assumptions are about 70% we're "sisters" and 30% "lesbians," whereas before we were always assumed to be married. Now, if we go out to dinner with a male friend, the waiter sometimes thinks one of us is married to HIM!
I like being seen as a lesbian, but I don't enjoy that we did lose that hetero privilege.