For me - there is a big definite switch between my male and female persona. It's my presentation. If I wear a dress (or blouse + skirt combo), have make up on my face (even minimal - lipstick), then I remove all my male inhibitions and expect not to be called sir. And when I am not doing it, there are no traces or hints of my feminine side, no slightest androgynity. I hate when someone calls me sir then (mostly - because it's so formal and I am neither in a position of power, nor that old), but not against less formal 'dude', 'man', 'mister', etc.
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