I would occasionally get male fail years before I started HRT. Deb and I were walking through the booths at Pride one year. I was wearing, jeans, sneakers, t-shirt, a white ball cap with my hair in a pony-tail and no makeup. When we passed the booth for the Feminist Women's Clinic, one of the women working the booth stepped out and addressed us as, "Ladies," while putting stickers on each of our shirts. The stickers read, "Carpe Boobum" (Seize the Boob), a reminder to do monthly breast exams.
Another time I had been helping a lesbian friend build a rail around her porch. We took a break to drive around to the corner store for a Coke. I was hot & dirty, soaking wet with perspiration and the only feminine thing about me at the time was a pair of gold ball earrings. When I walked into the store I passed three kids about 11-12 years old and I heard one of them say, "Hey, that was a lady!" much to the delight of my friend who got a big laugh out of it.