In word no, but what does that mean? Given that I still have a large amount of facial hair, I would never be taken for a woman, until I remove it. After that, we'll see.
Having adopted the philosophy of General Semantics many years ago, ( it had limited effect on my depression, but that it is to be expected) I believe we all have a self image, but the question is how accurate is it? In a way all our problems could be said to stem from this.
I am aware of the need to catalogue and date every observation, of myself and everything else. In other words, this is me now; not last week ;or yesterday; or 2 minutes ago .
How I interpret the reaction of others is dependent on my point of view, whether I think they are looking at me with scorn, or thinking about something else entirely is a different question. Perhaps the reaction of someone else again could tell me more about the first person's attitude. More data. Assuming they do not approach me directly I cannot make a definitive judgement.
To cut a long story short, unless people make their reaction plain, it's probably an even bet they haven't given your gender two thoughts.